UPDATE OF GROWTH AND NEW SEEDS

Cauliflower growth week 2 I think I was a bit to fast to throw away a few of my previous petri dishes and tubes that ‘looked’ contaminated. Above is a shot of some growth! this was from the first batch i made a few weeks back. New leaves have began to sprout and some pieces are growing roots.

Using seeds that haven’t been sterilised I wanted to know what happened if I used seeds that weren’t sterile - this growth took around 2 days! Good to know there is a real reason to sterilise the tissue before placing it on the medium.

Growing Tobacco seeds In on protocol I read through they began their experiment by growing the plant from seed and said that tobacco was best as its pretty hardy and grows fast. I decided, as i made so many MS dishes to experiment with this would be a good use of at least 3. I first sterilised the seeds but placing them in a bowl, in the sterile hood and pouring 70% ethanol over it. I did this 3 times, each time waiting ±20min for the ethanol to dry. I then stored them in sterile petri dish for safe keeping. As they are super tiny seeds one needs to be careful when sterilising them, which is why I chose to do it on tissue paper and ethanol rather than spraying or soaking them as I did with the other seeds.